β-NADPH-D-MAINTAIN BIOTECH Co.,Ltd.

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β-NADPH-D

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[(S)-4-d]NADPH CAS 74784-45-5 Full English Technical Datasheet

CAS No.: 74784-45-5
Full Name: β-Nicotinamide Adenine Dinucleotide Phosphate Reduced, (S)-4-deuterated form
Short Name: [(S)-4-d]NADPH, NADPH-d?, Deuterated NADPH
MDL No.: MFCD18252564
Synonyms: (S)-4-deuterio-1,4-dihydronicotinamide adenine dinucleotide phosphate, Labeled NADPH for mass spec
Molecular Formula: C??H??DN?O??P?
Molecular Weight: 746.43 g/mol
Isotope Feature: Single deuterium (2H) labeling at the C4-S position of the dihydropyridine ring of nicotinamide moiety

Test Item	Standard Specification
Appearance	Pale light yellow crystalline powder
Odor	Odorless
Solubility	Freely soluble in neutral/alkaline aqueous PBS buffer; soluble in DMSO; slightly soluble in methanol; insoluble in acetone, oils, alkanes
UV Characteristic Absorption	λmax 260 nm & 340 nm; Molar extinction coefficient ε??? = 6220 (pH 8.0–10.0), identical to unlabeled NADPH
A???/A??? Ratio	2.20 ~ 2.45
pH (0.5% aqueous solution)	9.0 ~ 11.0
Stability	Extremely light-sensitive, rapidly oxidized to NADP? under UV/acid/high temperature; stable under dark, alkaline, low-temperature vacuum sealed storage
Hygroscopicity	Highly hygroscopic, strictly vacuum moisture-proof packaging required

QC Item	Standard Limit	Test Method
Assay (HPLC, anhydrous basis)	≥95.0%	RP-HPLC UV 340 nm
Deuterium Isotope Enrichment	≥98 atom % D at C4-S site	LC-MS / NMR
Oxidized Impurity NADP?	≤3.00%	HPLC
Single Unknown Related Impurity	≤0.50%	HPLC
Total Related Impurities	≤1.50%	HPLC
Loss on Drying (KF)	≤7.0%	Karl Fischer Titration
Enzymatic Activity Verification	Pass G6PDH activity test	UV Spectrophotometry

Microbe Index	Standard
Total Aerobic Microbial Count	≤100 CFU/g
Yeast & Mold	≤10 CFU/g
Pathogens (E.coli, Salmonella, S. aureus)	Negative per 25g

Isotope-Labeled Reduced Coenzyme II Standard

Retains complete natural enzymatic binding activity of unlabeled NADPH; deuterium substitution only alters molecular mass without changing redox reactivity.
Gold Standard Substrate for Metabolic Tracing & LC-MS Quantification

Distinct mass shift (+1 Da) from native NADPH, enables absolute quantitative analysis of pentose phosphate pathway, redox metabolism, and biocatalytic reduction reactions via liquid chromatography-mass spectrometry.
Cofactor for Deuterium Transfer Mechanism Research

Used to clarify stereospecific hydride transfer (pro-R / pro-S) catalyzed by dehydrogenases, cytochrome P450 monooxygenases, reductases and oxidoreductases.
Internal Calibration Reference for Clinical Diagnostic Kits

Isotope internal standard for high-precision detection of NADPH/NADP? pools in cell lysate, serum, tissue samples.
Biocatalysis Deuterated Chiral Compound Synthesis Precursor

Supplies deuterated hydride for asymmetric reduction to produce stable isotope-labeled chiral drug intermediates.

Pentose phosphate pathway tracing, cellular redox metabolome absolute quantification, NAD(P) pool profiling
Formula Tip: Prepare stock solution in pH 8.0–10.0 alkaline buffer, avoid acidic dilution to prevent oxidation

Dehydrogenase hydride transfer stereospecificity study, G6PDH, 6PGDH, cytochrome P450 catalytic mechanism analysis

Chiral alcohol, steroid, amino acid deuterated derivatives bioreduction raw material

Isotope internal standard for antioxidant biomarker high-sensitivity quantitative assay

Irritant to skin, eyes and respiratory tract upon direct contact (GHS Xi hazard)
For laboratory research only; not for oral administration, cosmetic or human clinical direct use
No cytotoxicity under standard in-vitro biochemical working concentration
Lab operation requires nitrile gloves, safety goggles and dust mask

Storage Condition: Hermetic aluminum foil vacuum sealed; long-term storage at -20°C, absolute light-proof & moisture-proof; strictly avoid repeated freeze-thaw cycles; short-term storage ≤4°C
Shelf Life: 24 months under intact original vacuum packaging
Packaging: 10mg/50mg/100mg aluminum vacuum foil bags for lab mass spec research