β-Nicotinamide adenine dinucleotide phosphate Reduced form
- Model：1G,10G,1KG,5KG,25KG
- Createtime： 2026-07-02
- Updatetime： 2026-07-16
Product Details
| useage |
FOR R&D RESEARCH USE
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| deliveryInfo |
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| appearance |
white to off white powder
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| aliasen |
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| supplyCapacity |
50Tons/Year
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| harbor |
SHENZHEN
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| minorder |
1g
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β-NADPH (Free Acid) CAS 53-57-6 Full English Technical Datasheet
Important Distinction
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CAS 53-57-6: β-Nicotinamide Adenine Dinucleotide Phosphate Reduced (free acid, NADPH base)
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CAS 2646-71-1: NADPH Tetrasodium Salt (water-soluble sodium salt form, mainstream reagent grade)
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CAS 53-57-6 Nicotinamide = Niacinamide (Vitamin B3) is a different compound, do not confuse.
1. Basic Chemical Information
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CAS No.: 53-57-6PubChem
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Full Name: β-Nicotinamide Adenine Dinucleotide Phosphate, Reduced Form
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Abbreviation: β-NADPH / TPNH / Reduced Coenzyme II
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Synonyms: Dihydronicotinamide Adenine Dinucleotide Phosphate, Triphosphopyridine Nucleotide Reduced
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IUPAC Name: [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl ({[(2R,3S,4S)-5-(3-carbamoyl-1,4-dihydropyridin-1-yl)-2,3,4-trihydroxypentyl]phosphonato}oxy)phosphoric acid
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Molecular Formula: C??H??N?O??P?PubChem
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Molecular Weight: 745.42 g/mol
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EINECS: 200-177-6
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PubChem CID: 5884
2. Physical & Chemical Properties
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Test Item
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Standard Specification
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Appearance
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White to pale light yellow crystalline solid powder
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Odor
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Odorless
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Solubility
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Slightly soluble in neutral water; freely soluble in weak alkaline aqueous buffer; soluble in DMSO; insoluble in ethanol, acetone, oils
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UV Characteristic Absorption
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λmax 260 nm & 340 nm; Molar extinction coefficient ε??? = 6220 (pH 8.0–10.0)
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A???/A??? Ratio
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2.20 ~ 2.45
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pH (saturated aqueous suspension)
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3.0 ~ 4.5 (free acid, weak acidity)
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Stability
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Extremely light-sensitive; rapidly degraded under acidic, high temperature, UV irradiation; stable under dark, alkaline, low-temperature sealed storage
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Hygroscopicity
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Highly hygroscopic, must be stored in vacuum moisture-proof packaging
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3. Quality Specifications (Biochemical Research Grade)
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QC Item
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Standard Limit
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Test Method
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Assay (HPLC, anhydrous basis)
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≥97.0%; Premium ≥99.0%
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RP-HPLC UV 340 nm
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Loss on Drying (KF)
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≤7.0%
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Karl Fischer Titration
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Oxidized Impurity NADP?
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≤2.0%
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HPLC
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Single Unknown Related Impurity
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≤0.40%
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HPLC
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Total Related Impurities
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≤1.20%
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HPLC
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Enzymatic Activity Verification
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Pass glutathione reductase activity test
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UV Spectrophotometry
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Heavy Metals
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Heavy Metal
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Limit
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Lead (Pb)
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≤0.5 ppm
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Arsenic (As)
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≤1.0 ppm
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Cadmium (Cd)
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≤0.1 ppm
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Mercury (Hg)
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≤0.1 ppm
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Microbial Index
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Microbe Item
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Standard
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Total Aerobic Microbial Count
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≤500 CFU/g
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Yeast & Mold
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≤50 CFU/g
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Pathogens (E. coli, Salmonella, S. aureus)
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Negative per 25g
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4. Core Biochemical Mechanism & Biological Functions
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Primary Cellular Reducing Equivalent Cofactor
The core hydrogen donor for all anabolic biosynthesis; generated via pentose phosphate pathway, delivers hydride ions for synthetic metabolism.
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Biosynthesis Cofactor
Indispensable substrate for lipid, cholesterol, fatty acid, steroid, nucleic acid and amino acid synthesis; supports cytochrome P450 drug hydroxylation.
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Intracellular Antioxidant Defense Core
Regenerates reduced glutathione (GSH) via glutathione reductase; eliminates ROS free radicals, inhibits lipid peroxidation and oxidative cell damage.
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Immune Respiratory Burst Medium
Provides electrons for NADPH oxidase in macrophages to produce reactive oxygen species for pathogen clearance.
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Universal Enzymatic Assay Marker
340 nm absorbance signal is standard detection readout for dehydrogenases, reductases, NOS, liver function & clinical diagnostic kits.
5. Application Fields & Usage Tips
5.1 Biochemical Laboratory Research
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Enzyme kinetics, redox metabolism pathway, pentose phosphate pathway research
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Cell culture medium additive, biosensor raw material, in-vitro metabolic simulation
5.2 Clinical Diagnostic Reagent Raw Material
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Detection kits for glucose-6-phosphate dehydrogenase, glutathione reductase, blood lipid, liver antioxidant indicators
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Formula Tip: Prepare working solution with pH 8.0–10.0 alkaline buffer to improve solubility & stability
5.3 Pharmaceutical R&D Intermediate
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Anti-oxidation, mitochondrial protection new drug cell experimental raw material
5.4 High-End Cosmetic Raw Material
Anti-photoaging, energy replenishing antioxidant essence; recommended addition: 0.005%–0.02%; formula pH must be adjusted above 7.5 with alkaline solubilizer
6. Safety Profile
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Irritant to skin, eyes and respiratory tract upon direct contact (GHS Xi hazard)
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No cytotoxicity under standard in-vitro biochemical working concentration
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Not for direct oral administration; lab operation requires gloves, goggles and dust mask
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Non-sensitizing, non-phototoxic at low cosmetic dosage
7. Storage & Packaging
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Storage Condition: Hermetic vacuum foil sealed; long-term storage at -20°C, absolute light-proof & moisture-proof; avoid repeated freeze-thaw cycles; short-term storage ≤4°C
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Shelf Life: 24 months under intact original vacuum packaging
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Packaging: 100mg/500mg/1g/5g aluminum vacuum foil bags for lab research; 25kg double PE inner bag + paper drum for bulk factory order
