1. Core Product Specifications

Product Name: Nicotinic Acid Adenine Dinucleotide
Abbreviation: Deamido-NAD, ONAD
Alias: Desamido-NAD, Coenzyme I analog
CAS No.: 6450-77-7
Product Type: NAD+ structural analog, deamidated coenzyme derivative, cellular metabolic research reagent, enzyme substrate and inhibitor, biochemical pathway probe
Molecular Formula: C??H??N?O??P?
Molecular Weight: 664.41 g/mol
Structural Characteristics: Structural analog of Coenzyme I (NAD?), in which the amino group on the nicotinamide ring is replaced by a carboxyl group, forming a stable deamidated structure
Appearance: White to off-white crystalline powder, uniform loose texture, non-caking, odorless with ultra-low impurities
Purity: ≥99.0% (HPLC, Research Grade / Biochemical Grade)
Moisture Content: ≤2.0%
Heavy Metals: ≤10ppm
Residue on Ignition: ≤0.1%
Solubility: Freely soluble in pure water and aqueous buffer solutions, slightly soluble in ethanol. The solution is clear, stable and precipitation-free
Transport Condition: Sealed, dry and light-proof transportation at room temperature
Storage Condition: Long-term dry powder storage at -20℃ in sealed, dry and dark environment; short-term storage at 2–8℃. Avoid high temperature, humidity and strong light exposure to prevent molecular structure degradation and activity attenuation
Production Technology: Strict chemical semi-synthesis technology, structural deamidation modification, high-precision isomer separation, multi-stage HPLC refining, low-temperature vacuum drying activity locking technology
Core Bioactivity: NAD+ analog metabolic regulation, coenzyme competitive intervention, enzyme activity research, cellular redox mechanism exploration, deamidated coenzyme pathway research
Shelf Life: 24 months for sealed dry powder stored at -20℃ under standard dry and dark conditions
Usage Restriction: For scientific research, biochemical enzymology experiments and metabolic pathway mechanism research only. Strictly prohibited for unauthorized clinical use, food addition and illegal applications

2. Professional Product Introduction

Nicotinic Acid Adenine Dinucleotide (Deamido-NAD, ONAD, CAS: 6450-77-7) is a classic deamidated structural analog of natural NAD+ (Coenzyme I) and a key specific tool reagent in biochemical and metabolic research. Distinguished from standard NAD+, NMN, and NR series metabolites, Deamido-NAD features a unique modified structure where the amino group on the nicotinamide ring is replaced by a carboxyl group, forming a stable non-amidated coenzyme configuration. As a specific NAD+ analog, it can competitively bind with partial NAD+-dependent enzymes, effectively interfere with intracellular coenzyme metabolic pathways, and serve as an ideal control substrate and inhibitor for exploring coenzyme catalytic mechanisms, enzyme active site verification, and cellular redox regulation rules. With accurate molecular modification, stable structural activity and high biochemical specificity, it is widely applied in enzymology research, coenzyme structural simulation experiments, microbial metabolic pathway modification, and NAD+ functional differentiation mechanism studies.

3. Product Description

This product is high-purity research/biochemical-grade Nicotinic Acid Adenine Dinucleotide (Deamido-NAD/ONAD) crystalline powder, strictly synthesized and modified according to the official standard structure of CAS 6450-77-7. The product accurately completes nicotinamide ring deamidation and carboxylation modification, with pure target configuration, no unmodified NAD+ raw material residue, no invalid isomers and no synthetic impurity contamination. Multi-stage high-precision preparative HPLC deep refining thoroughly removes intermediate by-products and trace heterologous impurities, ensuring stable HPLC purity above 99.0% with ultra-high structural consistency and excellent experimental repeatability. The unique deamidated structure retains the basic skeleton of NAD+ coenzyme while changing the key functional groups, providing exclusive structural specificity for coenzyme competitive inhibition and enzymatic mechanism research. All quality indexes such as heavy metals, moisture and residue are strictly controlled, with ultra-low cytotoxicity and excellent biocompatibility. It is fully suitable for coenzyme-dependent enzyme kinetic experiments, intracellular redox intervention models, NAD+ structure-function relationship verification and synthetic biology modification research, meeting the strict quality requirements of high-end biochemical scientific research.

4. Core Product Advantages

1. Accurate Deamidated NAD+ Analog Structure
Strictly conforms to CAS 6450-77-7 standard structure, with precise replacement of nicotinamide ring amino group by carboxyl group. The structure is unique and pure, completely different from natural NAD+ and other NAD precursors, ensuring exclusive experimental targeting.

2. Professional Coenzyme Research Tool
As a classic competitive analog of NAD+, it can specifically interfere with coenzyme-dependent enzymatic reactions, providing irreplaceable experimental materials for studying coenzyme binding sites, catalytic mechanisms and metabolic pathway differentiation.

3. High Structural Stability & Experimental Repeatability
The modified deamidated-carboxylated structure has strong molecular stability, not easy to hydrolyze and degrade in aqueous solution. It maintains stable activity during long-term experiments, ensuring consistent and reliable experimental data.

4. Unique Academic Research Value
Deamido-NAD is a core specific reagent for NAD+ structure-function correlation research, with unique structural characteristics and clear mechanism of action. It is widely recognized in enzymology, metabolic biology and synthetic biology research fields.

5. Ultra-High Purity & Strict Quality Control
HPLC purity ≥99.0% with strictly controlled impurity residues and highly unified batch structure. It eliminates non-specific experimental interference and fully meets the requirements of high-precision enzymology and metabolic mechanism research.