Professional Product Parameters: CAS 151533-22-1 (Levomefolate Calcium / L?5?Methyltetrahydrofolate Calcium)

1. Basic Identification

• CAS No.：151533?22?1
• Product Name：Levomefolate Calcium; Calcium L?5?methyltetrahydrofolate (L?5?MTHF?Ca); 6S?5?Methyltetrahydrofolate Calcium; Active Folate
• IUPAC Name：L?Glutamic acid, N?[4?[[[(6S)?2?amino?3,4,5,6,7,8?hexahydro?5?methyl?4?oxo?6?pteridinyl]methyl]amino]benzoyl]?, calcium salt (1:1)FUJIFILM W...
• Synonyms：L?5?MTHF?Ca; 5?Methyltetrahydrofolate Calcium; Metafolin; Deplin
• MDL Number：MFCD00800468
• EINECS：691?636?3

2. Chemical Data

• Molecular Formula：C??H??CaN?O?
• Molecular Weight：497.52 g/mol
• Exact Mass：497.14 Da
• Elemental Analysis：C 48.28%, H 4.66%, Ca 8.04%, N 19.70%, O 19.30%
• Calculated LogP：?2.89
• TPSA：207.60 ?2
• Hydrogen Bond Donors：6
• Hydrogen Bond Acceptors：13
• Rotatable Bonds：9

3. Physical & Chemical Properties

• Appearance：White to off?white or light yellow crystalline powder; odorless
• Solubility：
  • Water：Sparingly soluble (≈0.5 mg/mL at 25°C; soluble when heated)
  • Aqueous acids：Soluble (when heated)
  • Aqueous alkalis：Soluble
  • Organic solvents (ethanol, methanol, DMSO, chloroform)：Very slightly soluble or insoluble
• Melting Point：>300 °C (decomposes)
• pH (1% aqueous suspension)：6.0–8.0
• Stability：Hygroscopic; sensitive to light, heat, and oxidation; stable under inert gas (N?/Ar) protection; avoid contact with strong acids/bases
• Storage Conditions：
  • Short?term (days–weeks): 2–8 °C, dry, light?proof, inert atmosphere
  • Long?term (months–years): ?20 °C, sealed, anhydrous, inert gas protection
• Shelf Life：24 months (under proper storage)

4. Product Specifications (Typical USP/Pharmaceutical Grade)

• Purity：≥99.0% (HPLC); ≥99.5% (Premium Grade)
• Single Maximum Impurity：≤0.5%
• Total Impurities：≤2.5%
• Related Impurities：
  • 5,10?Methylenetetrahydrofolic acid：≤0.5%
  • 5?Methyltetrahydropteroic acid：≤0.5%
  • Dimethyltetrahydrofolic acid：≤0.15%
• Calcium Content：7.8%–8.3% (calculated as Ca)
• Moisture：≤5.0%
• Heavy Metals：
  • Total：≤20 ppm
  • As：≤1.5 ppm; Cd：≤1.0 ppm; Pb：≤2.0 ppm; Hg：≤0.1 ppm; Pt：≤10 ppm
• Residue on Ignition：≤1.0%
• Particle Size：D50 = 10–30 μm (customizable)
• Endotoxin：≤5 EU/mg (for injectable grade)
• Grade：USP Grade; Pharmaceutical API; Food/ Dietary Supplement Grade; Research Grade
• Packaging：100 mg, 500 mg, 1 g, 5 g, 10 g, 25 kg (aluminum foil bag, fiber drum); custom packaging available

5. Biological Function & Applications

• Mechanism：
  • The biologically active form of folate (vitamin B9); structurally identical to endogenous 5?methyltetrahydrofolate (5?MTHF)
  • Directly participates in one?carbon transfer reactions for DNA synthesis, methylation, and homocysteine metabolism
  • Bypasses MTHFR enzyme deficiency; no DHFR?dependent activation required (unlike synthetic folic acid)
• Core Bioactivity：
  • Supports red blood cell formation and prevents megaloblastic anemia
  • Regulates homocysteine levels, supporting cardiovascular health
  • Essential for fetal neural tube development (pregnancy supplementation)
  • Improves folate status in MTHFR polymorphism carriers
• Applications：
  • Pharmaceutical API (e.g., Deplin® for folate deficiency, depression adjunct therapy)
  • Dietary supplements (pregnancy, elderly, MTHFR?deficient populations)
  • Fortified foods and functional beverages
  • Research tool for folate metabolism, epigenetics, and neuropsychiatric studies

6. Safety & Handling Instructions

• GHS Classification：Not classified as hazardous; no acute toxicity reported
• Handling：Avoid inhalation, skin/eye contact; use in well?ventilated area; wear gloves, goggles, and lab coat
• Disposal：Dispose in accordance with local environmental regulations for chemical waste
• Note：For pharmaceutical, food, and research use only; not for unauthorized human/animal administration